异源表达
异源的
生物
表达式(计算机科学)
多样性(政治)
计算生物学
进化生物学
遗传学
重组DNA
基因
计算机科学
社会学
人类学
程序设计语言
作者
Wei Liu,Shilan Zhai,Changsheng Zhang,Yuchan Chen,Zhiyong Liu,Wenqi Ma,Tianyu Zhang,Weiyang Zhang,Liang Ma,Changsheng Zhang,Wenjun Zhang
标识
DOI:10.1021/acs.jnatprod.3c01132
摘要
Thiazole scaffold-based small molecules exhibit a range of biological activities and play important roles in drug discovery. Based on bioinformatics analysis, a putative biosynthetic gene cluster (BGC) for thiazole-containing compounds was identified from Streptomyces sp. SCSIO 40020. Heterologous expression of this BGC led to the production of eight new thiazole-containing compounds, grisechelins E, F, and I–N (1, 2, 5–10), and two quinoline derivatives, grisechelins G and H (3 and 4). The structures of 1–10, including their absolute configurations, were elucidated by HRESIMS, NMR spectroscopic data, ECD calculations, and single-crystal X-ray diffraction analysis. Grisechelin F (2) is a unique derivative, distinguished by the presence of a salicylic acid moiety. The biosynthetic pathway for 2 was proposed based on bioinformatics analysis and in vivo gene knockout experiments. Grisechelin E (1) displayed moderate antimycobacterial activity against Mycobacterium tuberculosis H37Ra (MIC of 8 μg mL–1).
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