碳酸钙-2
生物信息学
大豆黄酮
大肠腺癌
体外
细胞毒性T细胞
结直肠癌
氟尿嘧啶
腺癌
细胞培养
染料木素
肿瘤科
内科学
医学
癌症研究
化学
药理学
生物
生物化学
癌症
遗传学
基因
作者
Altinay Altinkaynak,Orhan Koçak,Esra Tanyel Akçit,Ece Şimşek,Kübra Yıldırım,Ahmet Yılmaz Çoban
标识
DOI:10.1089/jmf.2024.0132
摘要
We aimed to evaluate the cytotoxic effect of daidzein alone and in combination with 5-fluorouracil (5-FU) on Caco-2 cells and investigate potential interactions between daidzein and 5-FU with apoptosis-related tumor necrosis factor-related apoptosis-inducing ligand receptor-2, tumor necrosis factor -alpha receptor-1 and Interferon gamma receptor-1. In this study, we used trypan blue and MTT assays to evaluate the cytotoxic effect of daidzein and the effect of daidzein on the amount of substance P released from the cells using the ELISA method. We utilized the ligand-protein molecular docking method for our in-silico assessments. According to our cytotoxicity experiment results, daidzein decreased the proliferation of Caco-2 cells. It should be pointed out that our ELISA test results daidzein and 5-FU increase the level of Substance-P (SP), a proinflammatory cytokine released by Caco-2 cells. Based on our in-silico results, daidzein interacted with all three apoptosis-related receptors on the membrane of Caco-2 cells to a better extent than 5-FU. In sum, although daidzein increases the amount of SP, it is a compound that has significant cytotoxic effect on colon cancer cells and promise as a potential therapeutic agent for the treatment of colon cancer.
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