生物
免疫系统
乳腺癌
免疫疗法
间质细胞
癌症研究
表观遗传学
N6-甲基腺苷
肿瘤微环境
生物标志物
癌症免疫疗法
渗透(HVAC)
基因签名
基因表达
癌症
免疫学
基因
甲基化
遗传学
物理
热力学
甲基转移酶
作者
Jian Zhang,Guihong Liu,Zili Dai,Fuchuan Xie,Ronghui Zheng,Benchao Yuan,Liyi Guo
出处
期刊:Gene
[Elsevier]
日期:2023-02-01
卷期号:853: 147095-147095
被引量:1
标识
DOI:10.1016/j.gene.2022.147095
摘要
Epigenetic mechanismshave been reported to involve in shaping tumor immune microenvironment (TME). However, the role of RNA N6-methyladenosine (m6A) modification in breast cancerhas not been fully explored.Based on m6A modification and TME infiltration characteristics of 2249 breast cancer patients, we comprehensively correlated m6A modification with immune landscapeby screeningcandidate genes, function analysis and constructing m6Asignatures. Principal component analysis was used to establish the m6Ascore. Both LASSO and Cox regression analyses were used to evaluate its prognostic value.Functional assays and immunohistochemistry were used to evaluate the expression of m6A regulators and immune cell infiltration.Based on the dysregulated expression of m6A, three distinct clusters were identified that displayed diverse types of tumour-associated TME cell infiltration in breast cancer.Gene signatures, stromal activity, and clinical prognosis were assessed by the m6Ascore. m6Ascore could function as a biomarker for predicting the therapeutic response to targeted therapy and immunotherapy.The dysregulated expression of m6Aregulators mediated the immune cell infiltration in the TME.Basedonthestudy,weidentified the signature and potential mechanism of m6AmodificationsthatmodifyTME cell infiltration. Thus, targeting m6A regulators may provide a promisingmethodoftreatingBRCA.
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