淋病奈瑟菌
多路复用
脑膜炎奈瑟菌
奈瑟菌
抗原
微生物学
脑膜炎球菌疫苗
奈瑟菌科
接种疫苗
细菌外膜
生物
病毒学
淋病
免疫分析
免疫学
抗体
细菌
大肠杆菌
免疫
抗生素
基因
生物信息学
遗传学
生物化学
人类免疫缺陷病毒(HIV)
作者
Erica Stover,Marguerite B Little,Kristie L. Connolly,Lixin Li,Robert A. Nicholas,Aleksandra E. Sikora,Ann E. Jerse,Marcia M. Hobbs,Joseph A. Duncan,Andrew N. Macintyre
标识
DOI:10.1093/infdis/jiae153
摘要
Abstract There is an urgent need for vaccines against Neisseria gonorrhoeae (Ng), the causative agent of gonorrhea. Vaccination with an outer-membrane vesicle (OMV)-based Neisseria meningitidis (Nm) vaccine provides some protection from Ng; however, the mechanisms underlying this cross-protection are unknown. To address this need, we developed multiplexed bead-based assays for the relative quantification of human and mouse IgG and IgA against Ng antigens. The assays were evaluated for analyte independence, dilutional linearity, specificity, sensitivity, intra- and inter-assay variability, and robustness to sample storage conditions. The assay was then used to test samples from mice and humans immunized with an Nm-OMV vaccine.
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