莫里斯水上航行任务
PI3K/AKT/mTOR通路
药理学
蛋白激酶B
神经保护
医学
突触可塑性
链脲佐菌素
海马体
内分泌学
内科学
糖尿病
化学
信号转导
受体
生物化学
作者
Longfei Li,Ying Gao,Yong Xu,Dan‐Jie Su,Qi Yang,An Liu,S.W. Wang,Xiuling Tang,Jingjing Zhao,Luo Lu,Tang Yan,Yumei Wu,Shui-bing Liu,Ming Zhao,Le Yang
摘要
Diabetic encephalopathy is a common consequence of diabetes mellitus that causes cognitive dysfunction and neuropsychiatric disorders. Praeruptorin C (Pra-C) from the traditional Chinese medicinal herb Peucedanum praeruptorum Dunn. is a potential antioxidant and neuroprotective agent. This study was conducted to investigate the molecular mechanisms underlying the effect of Pra-C on diabetic cognitive impairment. A novel object recognition test and the Morris water maze test were performed to assess the behavioral performance of mice. Electrophysiological recordings were made to monitor synaptic plasticity in the hippocampus. A protein-protein interaction network of putative Pra-C targets was constructed, and molecular docking simulations were performed to predict the potential mechanisms of the action of Pra-C. Protein expression levels were detected by western blotting. Pra-C administration significantly lowered body weight and fasting blood glucose levels and alleviated learning and memory deficits in type 2 diabetic mice. Network pharmacology and molecular docking results suggested that Pra-C affects the PI3K/AKT/GSK3β signaling pathway. Western blot analysis confirmed significant increases in phosphorylated PI3K, AKT, and GSK3β levels in vivo and in vitro upon Pra-C administration. Pra-C alleviated cognitive impairment in type 2 diabetic mice by activating PI3K/AKT/GSK3β pathway.
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