Doxorubicin‐Loaded Gold‐Hyaluronic Acid Nanoparticles for Postoperative Care of Breast Cancer Surgery

Abstract The study aims to prepare multifunctional nanotherapeutics to treat the problems that arise after surgery for breast cancer. This work proposes a postsurgical breast cancer depot system, the localized doxorubicin‐loaded gold‐hyaluronic acid nanoparticle (Dox‐Au‐HA‐NP), to address these problems. The ionic crosslinking was used to prepare the nanoparticles, which involved the interaction of cationic groups of gold (Au) with anionic hyaluronic acid (HA). The experiment design has been followed for selecting the concentration of experimental variables of nanoparticles. Optimized nanoparticles were characterized for their morphology, thermal analysis, antimicrobial study conducted in vitro, cytotoxicity, cell uptake, anti‐inflammatory, and matrix metalloproteinase (MMP) inhibitory potential. The prepared Dox‐Au‐HA‐NPs had an average particle size of 103.1 ± 0.2 nm, a polydispersity index of 0.264 ± 0.002, and an average zeta potential of −32.3 ± 0.2 mV. Encapsulation efficiency (EE) was found to be 92.2 ± 0.01%, and loading capacity (LC) was 12.73 ± 0.1%. The results of in vitro studies showed that the optimized nanoparticles outperformed the pure drug in terms of anticancer, anti‐inflammatory, and antimetastatic effects. The dox‐Au‐HA‐NP system represents a novel approach to improve postsurgical outcomes in breast cancer patients and presents a viable way to lower complications while enhancing efficacy.