Phytohabitans maris sp. nov., isolated from marine sediment

A novel actinobacterium, strain ZYX-F-186 T , was isolated from marine sediment sampled on Yongxing Island, Hainan Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain ZYX-F-186 T belongs to the genus Phytohabitans , with high similarity to Phytohabitans kaempferiae KK1-3 T (98.3 %), Phytohabitans rumicis K11-0047 T (98.1 %), Phytohabitans flavus K09-0627 T (98.1 %), Phytohabitans houttuyneae K11-0057 T (97.9 %), Phytohabitans suffuscus K07-0523 T (97.7 %), and Phytohabitans aurantiacus RD004123 T (97.7 %). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a single subclade in the genus Phytohabitans . The novel isolate contained meso -diaminopimelic acid, d -glutamic acid, glycine, d -alanine, and l -lysine in the cell wall. The whole-cell sugars were xylose, arabinose, ribose, and rhamnose. The predominant menaquinones were MK-9(H 8 ), MK-9(H 6 ), and MK-9(H 4 ). The characteristic phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The major fatty acids (>5 %) were iso-C 16 : 0 , anteiso-C 17 : 0 , and iso-C 18 : 0 . Genome sequencing showed a DNA G+C content of 71.9 mol%. Low average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity values demonstrated that strain ZYX-F-186 T could be readily distinguished from its closely related species. Based on its phylogenetic, chemotaxonomic, and physiological characteristics, strain ZYX-F-186 T represents a novel species of the genus Phytohabitans , for which the name Phytohabitans maris sp. nov. is proposed. The type strain is ZYX-F-186 T (=CGMCC 4.8025 T =CCTCC AA 2023025 T =JCM 36507 T ).