Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies

A novel platform for vaccines has been developed using self-assembling ferritin-based nanoparticles displaying influenza virus haemagglutinin; the haemagglutinin–nanoparticle vaccine induces more broad and potent neutralizing antibodies against diverse virus strains than a licensed influenza vaccine in mice and ferrets. The efficacy of the current generation of vaccines for seasonal influenza is limited by the need to produce new vaccines — using dated and time-consuming technologies — to cope with the rapidly evolving virus. This study presents a novel approach to influenza vaccination using self-assembling ferritin-based nanoparticles fused to the native viral attachment protein, haemagglutinin. The haemagglutinin–nanoparticle vaccine is shown to induce neutralizing antibodies and to generate higher immunity against diverse viral subtypes than a licensed influenza vaccine. For example, antibodies elicited by a 1999 haemagglutinin–nanoparticle vaccine neutralized H1N1 viruses from 1934 to 2007 and protected ferrets from infection by a 2007 H1N1 virus. Influenza viruses pose a significant threat to the public and are a burden on global health systems1,2. Each year, influenza vaccines must be rapidly produced to match circulating viruses, a process constrained by dated technology and vulnerable to unexpected strains emerging from humans and animal reservoirs. Here we use knowledge of protein structure to design self-assembling nanoparticles that elicit broader and more potent immunity than traditional influenza vaccines. The viral haemagglutinin was genetically fused to ferritin, a protein that naturally forms nanoparticles composed of 24 identical polypeptides3. Haemagglutinin was inserted at the interface of adjacent subunits so that it spontaneously assembled and generated eight trimeric viral spikes on its surface. Immunization with this influenza nanoparticle vaccine elicited haemagglutination inhibition antibody titres more than tenfold higher than those from the licensed inactivated vaccine. Furthermore, it elicited neutralizing antibodies to two highly conserved vulnerable haemagglutinin structures that are targets of universal vaccines: the stem4,5 and the receptor binding site on the head6,7. Antibodies elicited by a 1999 haemagglutinin–nanoparticle vaccine neutralized H1N1 viruses from 1934 to 2007 and protected ferrets from an unmatched 2007 H1N1 virus challenge. This structure-based, self-assembling synthetic nanoparticle vaccine improves the potency and breadth of influenza virus immunity, and it provides a foundation for building broader vaccine protection against emerging influenza viruses and other pathogens.