Congenital and acquired uterine anomalies are relatively often and correlate with infertility and habitual abortions. To estimate sensitivity and specificity of three-dimensional hysterosonosalpingography (3D HSSG) compared to hysteroscopy. 60 patients, divided into two groups, were included in this prospective study. In the first group, which consisted of 30 patients, estimates were done by 3D HSSG with negative contrast, and compared to findings of hysteroscopy done on the same patients. The second group, which also consisted of 30 patients, had uterine cavity estimation done by 3D HSSG with hyperechogenic contrast, and compared to finding of hysteroscopy done on these 30 women. Sensitivity and specificity of 3D HSSG with negative contrast was 100% compared to hysteroscopy. Sensitivity of 3D HSSG with hyperechogenic contrast compared to hysteroscopy was 66.6% for synechiae, 94.7% for septum and 100% for other uterine anomalies, while specificity was 100% for all uterine malformations. Results of this study show that 3D HSSG with negative contrast is the best method for uterine cavity vizualisation, and estimation, and it presents precise and minimally invasive alternative, that provides this method to be used in routine ambulatory practice.