The Suzuki-Miyaura coupling reaction catalyzed by Pd nanoparticles immobilized on widespread natural diatomite has been reported in this report. The reaction showed enhanced reaction rates, high yields, and the conditions were environmentally friendly. The highly active heterogeneous catalyst of palladium nanoparticles allow the reaction to be performed at low catalyst levels, and could be reused 5 times with little loss of activity. Transmission electron microscopy (TEM) and X-ray powder diffraction (XRD) analysis characteried the diatomite-supported Pd(0) nanoparticles as catalyst for the reaction.