Revealing the mechanisms of rhamnolipid enhanced hydrogen production from dark fermentation of waste activated sludge

Rhamnolipid (RL), as an environmentally compatible biosurfactant, has been used to enhance waste activated sludge (WAS) fermentation. However, the effect of RL on hydrogen accumulation in anaerobic fermentation remains unclear. Therefore, this work targets to investigate the mechanism of RL-based dark fermentation system on hydrogen production of WAS. It was found that the maximum yield of hydrogen increased from 1.76 ± 0.26 to 11.01 ± 0.30 mL/g VSS (volatile suspended solids), when RL concentration increased from 0 to 0.10 g/g TSS (total suspended solids). Further enhancement of RL level to 0.12 g/g TSS slightly reduced the production to 10.80 ± 0.28 mL/g VSS. Experimental findings revealed that although RL could be degraded to generate hydrogen, it did not play a major role in enhancing hydrogen accumulation. Mechanism analysis suggested that RL decreased the surface tension between sludge liquid and hydrophobic compounds, thus accelerating the solubilization of WAS, improving the proportion of biodegradable substances which could be used for subsequent hydrogen production. Regardless of the fact that adding RL suppressed all the fermentation processes, the inhibition effect of processes associated with hydrogen consumption was much severer than that of hydrogen production. Further investigations of microbial community revealed that RL enriched the relative abundance of hydrogen producers e.g., Romboutsia but reduced that of hydrogen consumers like Desulfobulbus and Caldisericum.