The study assessed the level of resistance against common fluoroquinolone antibiotics (ciprofloxacin, norfloxacin, ofloxacin and enrofloxacin), in bacterial species isolated from open wounds of domestic animals. Among the four fluoroquinolone antibiotics, the bacterial population showed maximal resistance against norfloxacin, only 25.56% of bacterial isolates were sensitive to this antibiotic. Ciprofloxacin, enrofloxacin and ofloxacin were almost similar in their efficacy against the bacterial isolates but 34–36% bacterial isolates were resistant to these antibiotics. The results indicated high bacterial resistance among fluoroquinolone antibiotics suggesting judicious use of this group of antibiotics under field conditions.