Iminocoumarin-based fluorescence probe for intracellular H2O2 detection with a red emission and a large Stokes shift

A highly sensitive and selective fluorescence probe for H2O2 has been developed based on an iminocoumarin derivative using aromatic boronic ester as sensing unit. A significant fluorescence enhancement was observed with λmaxem = 619 nm when this probe was treated with H2O2. In the detection of H2O2, this probe exhibited a large Stokes shift (148 nm) and a low detection limit (6.0 × 10−8 mol/L). Furthermore, cell imaging studies had revealed that this probe had the potential to detect intracellular H2O2 in living cells.