Nanoparticle-siRNA complexes inhibit SOCS1 gene expression on cord blood dendritic cells

Objective:To construct nanoparticle complexes and delivery siRNA sequence so as to inhibit SOCS1 gene expression on cord blood dendritic cells.Methods: Synthesized nanoparticle complexes using SiO2 and Fe3O4 enveloped by PEI.Using LipofectamineTM2000 as control,agarose gel electrophoresis analyzed the efficiency of nanoparticle-siRNA combination;Fluorescence microscopy and flow cytometry examined the cellular uptake of nanoparticle-siRNA complexes;Western blot assay detected the expression of SOCS1 protein before and/or after DCs were treated with nanoparticle-siRNA complexes;MTT assay evaluated the antitumor activities of effector cells stimulated by DCs.Results: Compared with the control group,nanoparticle complexes can efficiently combine the siRNA sequence.Nanoparticle-siRNA complexes were uptaked by cord blood dendritic cells.However,DCs treated with nanoparticle complexes alone insignificantly inhibit SOCS1 gene expression;in addition to the external magnetic field,SOCS1 gene expression were significantly reduced,and paralleled with the enhanced antitumor activities of effector cells stimulated by DCs.Conclusion: Nanoparticle complexes showed safety and high efficiency in siRNA sequence delivery in vitro.The expression of SOCS1 gene on cord blood dendritic cells were significantly silenced by nanoparticle-siRNA complexes.The results presented in this study provided a new way of thinking and experimental basis,which are expected to be useful for designing more effective dendritic cell-based antitumor immunotherapeutic strategies.