A study on extraction and isolation of total flavonoids from E laeagnus mollis leaves by macroporous absorptive resins

Through the stable absorption capacity and eluant effect selection, the LSA一10 resin is selected from five m acroporous absorptive resins. And it is the best suitable to the absorption and separation of total flavonoids from E laeaynus mollis leaves. The absorption process of total flavonoids from E.m o l lis leaves by this resin was researched, the results are as follows:(1)E luant is 50%一70% ethanol;(2) W hen the pH value of sample solution arrives at four, the absorption capacity reaches highest;(3)With the time improving, the absorption capacity is rising gradually. The optimum time of absorption is 8一10 h;(4) W ith the capacityration rising, the absorption capacity reaches higher, but the concentration shouldn't be so high, the concentration of sample solution should reach the clarification.