Cytological Studies on Anther Development of Non-1B/1R Wheat Male Sterile Lines

In order to study the change of anther structure of non-1B/1R wheat male sterile lines at the stage of pollen abortion,the anther development of non-1B/1R wheat male sterile line 732A and its maintainer line 732B were observed by TEM.The results showed that tapetum tissue in anther of sterile lines deteriorated rapidly in the single nucleus pollen grain stage,the complete middle layer still could be seen in the two nuclear pollen grains phase.The disintegration of tapetum tissue was slow in anther of maintainer lines,the symptoms were disappeared completely until three nuclear pollen grains phase,but middle layer were disappeared completely in the sin-gle nucleus pollen grain stage.Therefore,tapetum is related to male abortion,middle layer cell can keep its completeness all the time,this is perhaps as a result of rapid degradation of tapetum.