We studied the laccase of polyporus in the curve of laccase production and optimal condition of laccase reaction. The result showed that polyporus came the laccase production peak which was 412u/mL at the 9th day in this culture condition The optimum temperature and optimum pH of laccase reaction were 25℃ and 4.2.Mg2+and Mn2+increased laccase activity whereas Ag+?Fe3+and Clobviously inhibited it.