Determination of Aucubin in Eucommia ulmoides bark from different origin by RP-HPLC

Objective:To determine the contents of aucubin in Eucommia ulmoides bark from different origin.Methods:The quantitative analysis was carried out with RP-HPLC on a Diamonsil C18 column(5 μm,150 mm×4.6 mm) with temperature at 15 ℃,the mobile phase was methanol-water(6:94) at 1 mL /min,and detection wavelength was 206 nm.Results: The linear ranges were 0.1656～3.312μg,The average recovery were 99.24 %,the relative standard deviations(RSD) were 1.74%.Conclusion: The method is proved to be specific and accurate.It can be used to determine the contents of aucubin in Eucommia ulmoides bark from different origin.