癌症研究
PI3K/AKT/mTOR通路
ROR1型
弥漫性大B细胞淋巴瘤
蛋白激酶B
基因敲除
细胞生长
医学
受体酪氨酸激酶
癌症
信号转导
淋巴瘤
生物
内科学
细胞凋亡
受体
细胞生物学
血小板源性生长因子受体
生长因子
生物化学
遗传学
作者
Man Yuan,Li Xu,Jun Wang,Louqian Zhang,Nan Hou,Jing Xu,Lin Wang,Shu Yang,Chen Yan,Lin Xiong,Jin Zhu,Weifei Fan,Jiaren Xu
出处
期刊:Biofactors
[Wiley]
日期:2019-02-24
卷期号:45 (3): 416-426
被引量:20
摘要
Abstract The receptor‐tyrosine‐kinase (RTK)‐like orphan receptor 1 (ROR1) is a transmembrane glycoprotein regarded as a tumor‐associated antigen. ROR1 plays an important role in cancer development, but the detailed function of ROR1 in diffuse large B‐cell lymphoma (DLBCL) remains unclear. In this study, we first detected ROR1 expression and evaluated the relationship between ROR1 expression and the clinicopathological characteristics of DLBCL patients. Next we employed shRNA‐mediated knockdown of ROR1 in DLBCL cell line to explore the characteristics of ROR1 in DLBCL development both in vitro and in vivo . The results showed a significantly higher level of ROR1 in DLBCL tissues than in lymphatic hyperplasia tissues. High ROR1 expression was correlated with unfavorable prognosis in DLBCL patients. Furthermore, ROR1 knockdown inhibited the growth and induced the apoptosis in DLBCL cells and xenografts. In addition, shROR1 inhibited activation of the PI3K/Akt/mTOR signaling pathway, both in vitro and in vivo . Taken together, our results suggest that ROR1 is a novel prognostic marker for DLBCL survival and ROR1 significantly promotes DLBCL tumorigenesis by regulating the PI3K/Akt/mTOR signaling pathway. Targeting ROR1 may provide a promising strategy for DLBCL treatment. © 2019 BioFactors, 45(3):416–426, 2019
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