浆液性液体
阶段(地层学)
病理
免疫组织化学
癌变
原位杂交
表皮生长因子受体
浆液性癌
卵巢癌
基因复制
荧光原位杂交
表皮生长因子
基因表达
卵巢癌
清除单元格
生物
医学
基因
癌症研究
癌症
受体
古生物学
生物化学
遗传学
染色体
作者
Julian Crasta,Gayatri Ravikumar,Savitha Rajarajan,Sumangala Gali,Kiran Abhijit Kulkarni,Elizabeth Vallikad,Jyothi S Prabhu
出处
期刊:International Journal of Gynecological Pathology
[Ovid Technologies (Wolters Kluwer)]
日期:2020-04-13
卷期号:40 (1): 49-55
被引量:2
标识
DOI:10.1097/pgp.0000000000000678
摘要
Human epidermal growth factors play an important role in ovarian carcinogenesis and are evaluated for prognostic and possible therapeutic roles in high-grade serous ovarian malignancies. The present study was undertaken to evaluate the expression of human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor (EGFR) in advanced stage serous carcinoma and their influence on prognosis. The expression of HER2 and EGFR was studied in 59 cases of stage III and IV ovarian serous carcinomas by immunohistochemistry and fluorescent in situ hybridization. Of the 48 interpretable tumors for HER2, 6 tumors (12.5%) were scored as positive, 14 (29%) as equivocal and 28 tumors (58.5%) were negative by immunohistochemistry, while only 2/48 (4%) showed frank amplification by fluorescent in situ hybridization with ≥4 copies per cell. HER2 gene expression measured by quantitative polymerase chain reaction had good positive correlation with both protein expression and gene amplification. Although EGFR expression was seen in 32% of tumors, none of the tumors positive for HER2 protein or gene amplification had co-expression of EGFR indicating mutual exclusivity of their expression. Gene expression of both proteins also confirmed their inverse correlation (Pearsons CC=−0.15, P =0.3). Further there was no influence of protein or gene expression of these markers on the overall survival. In conclusion, HER2 and EGFR are expressed in a small percentage of tumors and the mutual exclusivity of these markers precludes the possibility of dual targeting with anti-HER2 and anti-EGFR therapy in advanced stage high-grade serous ovarian carcinoma.
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