DNA
配体(生物化学)
拓扑(电路)
分子识别
化学
拓扑排序
细胞
计算生物学
受体
生物
分子
计算机科学
生物化学
数学
组合数学
有向无环图
有机化学
算法
作者
Fangfei Yin,Min Li,Xiuhai Mao,Fan Li,Xuelin Xiang,Qian Li,Lihua Wang,Xiaolei Zuo,Chunhai Fan,Ying Zhu
标识
DOI:10.1002/anie.202002020
摘要
Abstract Molecular recognition in cell biological process is characterized with specific locks‐and‐keys interactions between ligands and receptors, which are ubiquitously distributed on cell membrane with topological clustering. Few topologically‐engineered ligand systems enable the exploration of the binding strength between ligand‐receptor topological organization. Herein, we generate topologically controlled ligands by developing a family of tetrahedral DNA frameworks (TDFs), so the multiple ligands are stoichiometrically and topologically arranged. This topological control of multiple ligands changes the nature of the molecular recognition by inducing the receptor clustering, so the binding strength is significantly improved (ca. 10‐fold). The precise engineering of topological complexes formed by the TDFs are readily translated into effective binding control for cell patterning and binding strength control of cells for cell sorting. This work paves the way for the development of versatile design of topological ligands.
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