小火焰菌属
多糖
化学
指纹(计算)
高效液相色谱法
鼠李糖
色谱法
木糖
单糖
糖
半乳糖
葡萄糖醛酸
食品科学
生物化学
发酵
人工智能
蘑菇
计算机科学
作者
Yutong Dong,Fei Pei,Anxiang Su,Katherine Z. Sanidad,Gaoxing Ma,Liyan Zhao,Qiuhui Hu
标识
DOI:10.1016/j.fct.2019.110944
摘要
Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes. However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources. In this study, a multiple fingerprint analysis method including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography (HPLC) was established. The inhibitory activities of FVPs against HepG2 were measured and introduced into multiple linear regression (MLR) analysis to investigate fingerprint-activity relationship. The principal component analysis (PCA) scores showed that the polysaccharides extracted from 20 batches of different F. velutipes were highly similar, and substandard samples could be distinguished from the authentic polysaccharides clearly. The glucuronic acid could be considered as a marker for discrimination of white and yellow F. velutipes polysaccharides in HPLC fingerprints. Moreover, the HPLC fingerprint-growth inhibitory activity relationship illuminated that monosaccharides composition played an important role on the HepG2 growth inhibitory activity, and activity-associated markers (mannose, rhamnose, xylose, and galactose) were chosen to assess FVPs from different sources. The suggested HPLC fingerprint-activity relationship method provides an integrated strategy for the quality control of F. velutipes and its related products.
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