氯化十六烷基吡啶
脂磷壁酸
化学
三氯生
氟化物
脂多糖
类胡萝卜素
抗菌剂
微生物学
生物化学
抗菌肽
金黄色葡萄球菌
细菌
有机化学
无机化学
肺表面活性物质
生物
免疫学
医学
遗传学
病理
作者
John Christian Haught,Sancai Xie,Ben Circello,Cheryl S Tansky,Deepa Khambe,Yiping Sun,Yong Lin,K. Sreekrishna,Małgorzata Klukowska,Tom Huggins,Donald J White
出处
期刊:PubMed
日期:2016-12-01
卷期号:29 (6): 328-332
被引量:7
摘要
To study the reactivity of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) with the cationically charged agents cetylpyridinium chloride, stannous fluoride, and the non-cationic agent triclosan. We also assessed the effect of these agents to inhibit LPS and LTA binding to cellular Toll-like Receptors (TLRs) in vitro.The ability of these antimicrobials to bind with LPS and/or LTA was assessed in both the Limulus amebocyte lysate and BODIPY-TR-cadaverine dye assays. Mass spectroscopy was then used to confirm that stannous fluoride directly binds with LPS and to determine stoichiometry. Lastly, we looked for possible inhibitory effects of these antimicrobial agents on the ability of fluorescently conjugated LPS to bind to TLR4 expressed on HEK 293 cells.Cetylpyridinium chloride (CPC) and stannous salts including stannous fluoride interfered with LPS and LTA reactivity in both dye assays, while triclosan had no effect. Mass spectroscopy revealed direct binding of stannous fluoride with E. Coli LPS at 1:1 stoichiometric ratios. In the cellular assay, cetylpyridinium chloride and stannous fluoride, but not triclosan, inhibited LPS binding to TLR4.These results support a potential mechanism of action for stannous fluoride and CPC formulated in oral products in which these ingredients bind bacterial toxins and potentially render them less toxic to the host. These results may influence home care recommendations for patients at risk for plaque-related diseases.
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