Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States

生物 病毒学 开放式参考框架 戊型肝炎病毒 基因 遗传学 病毒 序列分析 打开阅读框 肽序列 基因型
作者
Gholamreza Haqshenas,H. L. Shivaprasad,Peter R. Woolcock,Deryck H. Read,Xiang‐Jin Meng
出处
期刊:Journal of General Virology [Microbiology Society]
卷期号:82 (10): 2449-2462 被引量:356
标识
DOI:10.1099/0022-1317-82-10-2449
摘要

Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the genetic identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. Phylogenetic analysis indicates that avian HEV is genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
宇心完成签到,获得积分10
2秒前
2秒前
郭666完成签到,获得积分20
3秒前
风趣的元气完成签到,获得积分10
3秒前
1111完成签到 ,获得积分10
4秒前
迟安歌给迟安歌的求助进行了留言
4秒前
Kara完成签到 ,获得积分10
4秒前
Dotson发布了新的文献求助10
4秒前
文静的翠安完成签到,获得积分10
4秒前
刘豆发布了新的文献求助30
4秒前
5秒前
昏睡的柜子完成签到,获得积分10
6秒前
Mercy发布了新的文献求助10
6秒前
Norah发布了新的文献求助10
6秒前
NexusExplorer应助鼓励男孩采纳,获得10
6秒前
夜猫完成签到,获得积分10
7秒前
大胆笑翠完成签到,获得积分10
7秒前
星辰大海应助tangz采纳,获得10
8秒前
8秒前
郁树春应助提笔写未来C采纳,获得10
9秒前
9秒前
大力的迎松完成签到,获得积分10
9秒前
不想干活应助郭666采纳,获得10
10秒前
凉风送信完成签到,获得积分10
10秒前
10秒前
研友_8KXdRL完成签到,获得积分10
10秒前
cliff139完成签到,获得积分10
10秒前
丹尼完成签到 ,获得积分10
11秒前
11秒前
11秒前
灵宝宝完成签到,获得积分10
12秒前
落花生完成签到,获得积分10
12秒前
13秒前
13秒前
tudouning完成签到,获得积分20
13秒前
共享精神应助青鸢采纳,获得10
13秒前
黑糖珍珠完成签到 ,获得积分10
14秒前
Dotson完成签到,获得积分10
14秒前
爆米花应助Mercy采纳,获得10
14秒前
高分求助中
(禁止应助)【重要!!请各位详细阅读】【科研通的精品贴汇总】 10000
International Code of Nomenclature for algae, fungi, and plants (Madrid Code) (Regnum Vegetabile) 1500
Robot-supported joining of reinforcement textiles with one-sided sewing heads 820
Византийско-аланские отно- шения (VI–XII вв.) 500
Improvement of Fingering-Induced Pattern Collapse by Adjusting Chemical Mixing Procedure 500
水稻光合CO2浓缩机制的创建及其作用研究 500
Logical form: From GB to Minimalism 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4179034
求助须知:如何正确求助?哪些是违规求助? 3714405
关于积分的说明 11710118
捐赠科研通 3395446
什么是DOI,文献DOI怎么找? 1862845
邀请新用户注册赠送积分活动 921488
科研通“疑难数据库(出版商)”最低求助积分说明 833299