Zinc ions-modified trypsin nanoparticles as fluorescent probe for rapid detection of tetracyclines antibiotics

Tetracyclines (TCs) are one of the most common antibiotics against bacterial infection, and the misuse of TCs has become a public problem affecting human health. Herein, we reported trypsin nanoparticles (Try NPs) with fluorescence emission peak at 445 nm and further modified Try NPs with Zn2+ ion to prepare Try NPs-Zn2+ complex. More importantly, Try NPs-Zn2+ displayed significant fluorescence response to multiple TCs including tetracycline, oxytetracycline, and chlorotetracycline at 525 nm in 30 s with 360 nm excitation wavelength, which can be used for rapid detection of TCs antibiotics.