光致发光
乙二醇
量子产额
光谱学
猝灭(荧光)
分析化学(期刊)
荧光光谱法
材料科学
光致发光激发
化学
吸收光谱法
荧光
光化学
有机化学
光电子学
光学
物理
量子力学
作者
Krishanan Bisht,Ruma Dutta
标识
DOI:10.1016/j.dyepig.2022.110823
摘要
Here we present l-valine functionalized non-graphitic carbon dots (denoted as N-CDvaline) as a photoluminescent (PL) probe for highly sensitive and selective detection of tetracycline antibiotic. The probe, synthesized by heating of ethylene glycol with l-valine at 170 °C for 10 min, exhibited bright bluish photoluminescence at λemission = 445 nm by exciting at λexcitation = 370 nm with 11.4% photoluminescence quantum yield. The higher resolution transmission electron micrograph, selected area electron diffraction, Fourier transformed infrared spectroscopy and X-ray photoelectron spectroscopy of N-CDvaline suggested highly ordered arrangement of linear transverse chains of oligomers of ethylene glycol and l-valine, attributable to self-assembly. Under optimized conditions, the probe exhibited linear PL quenching by tetracycline in the concentration range between 0.25 and 5.00 μM and satisfied the Stern-Volmer equation, with a detection limit of 0.054 μM. This method is highly selective towards tetracycline detection against host of interfering agents e.g., some common cations, anions and other classes of antibiotics. The PL quenching mechanism is attributed to ground state complex formation between the probe and tetracycline, corroborated by time resolved fluorescence spectroscopy studies and due to inner filter effect (IFE) owing to strong spectral overlap between UV–vis absorption spectrum of tetracycline with excitation and emission spectra of N-CDvaline. The scope for using this method for detection of tetracycline in real samples e.g., river water and milk is successfully demonstrated by spike analysis.
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