纳米毒理学
表观遗传学
增强子
组蛋白
化学
表观遗传学
细胞生物学
生物物理学
H3K4me3
转录组
染色质
转录因子
基因表达
纳米颗粒
计算生物学
纳米技术
生物
基因
材料科学
生物化学
发起人
DNA甲基化
作者
Federica Gamberoni,Marina Borgese,Christina Pagiatakis,Ilaria Armenia,Valeria Grazú,Rosalba Gornati,Simone Serio,Roberto Papait,Giovanni Bernardini
出处
期刊:Nano Letters
[American Chemical Society]
日期:2023-07-26
卷期号:23 (19): 9151-9159
标识
DOI:10.1021/acs.nanolett.3c01967
摘要
Despite the progress in the field of nanotoxicology, much about the cellular mechanisms that mediate the adverse effects of nanoparticles (NPs) and, in particular, the possible role of epigenetics in nanotoxicity, remains to be clarified. Therefore, we studied the changes occurring in the genome-wide distribution of H3K27ac, H3K4me1, H3K9me2, and H3K27me3 histone modifications and compared them with the transcriptome after exposing NIH3T3 cells to iron-based magnetic NPs (i.e., Fe2O3 and Fe2O3@Co NPs). We found that the transcription response is mainly due to changes in the genomic distribution of H3K27ac that can modulate the activity of enhancers. We propose that alteration of the epigenetic landscape is a key mechanism in defining the gene expression program changes resulting in nanotoxicity. With this approach, it is possible to construct a data set of genomic regions that could be useful for defining toxicity in a manner that is more comprehensive than what is possible with the present toxicology assays.
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