JoVE Video Dataset

777 Views. King Abdullah University of Science and Technology (KAUST). Establishing a ChIP-seq protocol in Aiptasia is the first step in investigating the epigenetic landmarks in symbiotic cnidarians, and how these may regulate an organism's interaction with its environment. The protocol is optimized to address challenging cnidarian features, such as high mucus production and a high water-to-tissue ratio, by increasing the cross-linking time and minimizing tissue loss. Collect the anemones in a 15-milliliter tube and let them settle to the bottom of the tube.