Intelligent Carbon Dots with Switchable Photo‐Activated Oxidase‐Mimicking Activity and pH Responsive Antioxidant Activity Adaptive to the Wound Microenvironment for Selective Antibacterial Therapy

Intelligent antibacterial agent with controllable activities adaptive to the wound microenvironment is appealing to reduce drug resistance and enhance antibacterial efficiency. In this study, celery is chosen as the carbon source to construct celery-based carbon dots (CECDs) with double activities, i.e., reactive oxygen species (ROS)-production and ROS-clearance activities. The ROS-production capability of CECDs is dependent on the oxidase (OXD)-mimicking activity, which is only photo-activated and thus artificially controlled by light to avoid the production of excess ROS. Meanwhile, the optimal OXD-mimicking activity occurrs at the pH of 5, close to microenvironmental pH at the bacterial infection site, which will enhance the antibacterial efficacy. On the other hand, CECDs exhibit the antioxidant activity at the neutral or weak alkaline pH, which will assist the healing of the wound. Thus, the conversion of ROS-production and ROS-clearance ability of CECDs can be dynamically and intelligently switched automatically with microenvironmental pH at different stages of treatment (from acid to neutral/weak basic). The proposed CECDs exert adorable selective antibacterial activity against Gram-positive bacteria and satisfactory therapeutic effect on bacteria infected mice. This study paves a new avenue to design the intelligent antibacterial nanoagent sensitive to the infected microenvironmental condition, reducing drug resistance and assisting precise medicine.