A ratiometric fluorescence platform based on WS2 QDs/CoOOH nanosheet system for α-glucosidase activity detection

In this study, we have constructed a ratiometric fluorescence sensor for sensitive sensing of α-glucosidase activity based on WS2 QDs/ CoOOH nanosheet system. In this system, as an oxidase-imimicking nanomaterial, CoOOH nanosheet could convert o-phenylenediamine into 2,3-diaminophenazine (DAP), which had a high fluorescence emission at 575 nm. The DAP subsequently could quench the fluorescence of WS2 QDs via the inner filter effect (IFE). L-Ascorbic acid-2-O-α-D-glucopyranose could be hydrolyzed by α-glucosidase to yield ascorbic acid. CoOOH nanosheet can be converted to Co2+ ions by ascorbic acid, leading to the fluorescence decrease of DAP and the fluorescence recovery of WS2 QDs. Therefore, a novel ratio fluorescence sensing strategy was established for α-glucosidase detection based on WS2 QDs/CoOOH nanosheet system. This WS2 QDs/CoOOH nanosheet system has a low detection limit of 0.009 U/mL for α-Glu assay. The proposed strategy succeeded in detecting α-Glu in human serum samples.