Pharmacokinetics of Anti-rheumatic Drugs Methotrexate andTofacitinib with its Metabolite M9 in Rats by UPLC-MS/MS

The currently established analytical method exhibited great potential for the evaluation of plasma concentrations of methotrexate, tofacitinib and M9 simultaneously, greatly reducing the detection time, which would serve as a supplementary role in formulating dose decisions to achieve personalized treatment, identify drugs that cause adverse reactions and finally, to assess drug-drug interactions on clinical studies.