抗坏血酸
微电极
体内
谷氨酸受体
生物物理学
化学
药理学
材料科学
生物化学
医学
受体
生物
电极
食品科学
物理化学
生物技术
作者
Zexuan Meng,Yuchan Zhang,Yang Lu,Shuang Zhao,Qiang Zhou,Jiajia Chen,Jiuxi Sui,Jian Wang,Leilei Guo,Luyue Chang,Jialing He,Guixue Wang,Guangchao Zang
出处
期刊:Research
[AAAS00]
日期:2023-01-01
卷期号:6
被引量:1
标识
DOI:10.34133/research.0149
摘要
Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research, its application is limited by substantial interference in complex brain environments while ensuring biosafety requirements. In this study, we introduced poly(3-hexylthiophene) (P3HT) and nitrogen-doped multiwalled carbon nanotubes (N-MWCNTs) to construct a composite membrane-modified carbon fiber microelectrode (CFME/P3HT-N-MWCNTs) for ascorbic acid (AA) detection. The microelectrode presented good linearity, selectivity, stability, antifouling, and biocompatibility and exhibited great performance for application in neuroelectrochemical sensing. Subsequently, we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells, ex vivo brain slices, and in vivo living rat brains and determined that glutamate can induce cell edema and AA release. We also found that glutamate activated the N-methyl-d-aspartic acid receptor, which enhanced Na+ and Cl- inflow to induce osmotic stress, resulting in cytotoxic edema and ultimately AA release. This study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the mechanism. Our work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals, understand the molecular basis of nervous system diseases, and discover certain biomarkers of brain diseases.
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