OmicsCam Enables Trimodal Profiling of Mitochondrial Genome Editing

Mitochondrial DNA (mtDNA) editing can generate cellular and animal models of mitochondrial genetic disorders and holds promise for future ex vivo and in vivo therapeutic applications. However, due to the quantitative nature of mitochondrion genetics, as more base-editing tools evolve, it is crucial to evaluate not only their efficiency and specificity on the sequence level but also the resulting molecular phenotypes. Here, we devised a novel Omics Carrier microcapsule, abbreviated as OmicsCam, that achieves homogeneous reactions within a heterogeneous carrier membrane, enabling highly efficient multistep biochemistry workflows. Incorporating magnetic beads into the carrier enables high-throughput automation. We demonstrated simultaneous trimodal assessment of mtDNA editing