连接器
胶束
化学
膜
膜蛋白
生物物理学
功能(生物学)
脚手架
生物化学
有机化学
计算机科学
生物
细胞生物学
数据库
水溶液
操作系统
作者
Weiling Luo,Meifang Yang,Yitian Zhao,Huixia Judy Wang,Xiaodi Yang,Wei Zhang,Fei Zhao,Suwen Zhao,Houchao Tao
标识
DOI:10.1002/chem.202202242
摘要
It is a pressing need, but still challenging to explore the structure and function of membrane proteins (MPs). One of the main obstacles is the limited availability of matched detergents for the handling of specific MPs. We describe herein the design of new detergents by incorporation of a transition linker between the hydrophilic head and the hydrophobic tail. This design allows a gradual change of hydrophobicity between the outside and inside of micelles, in contrast to the abrupt switch in conventional detergents. Notably, many of these detergents assembled into micelles in while retaining low critical micelle concentrations. Meanwhile, thermal stabilizing evaluation identified superior detergents for representative MPs, including G protein-coupled receptors and a transporter protein. Among them, further improved the NMR study of MPs. We anticipate these that results will encourage future detergent expansion through new remodeling on the traditional detergent scaffold.
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