Soil multifunctionality of paddy field is explained by soil pH rather than microbial diversity after 8-years of repeated applications of biochar and nitrogen fertilizer

Biochar and nitrogen (N) fertilizer application can increase soil carbon sequestration and enhance soil nutrient cycling. However, few studies have systematically explored the effects of the long-term application of biochar and N fertilizer on soil multifunctionality and characterized its driving factors. Based on an 8-year biochar paddy-field experiment in anthropogenic alluvial alkaline soil in northwest China, we measured eleven soil functions associated with soil carbon sequestration and nutrient cycling and four potential factors (soil bacterial and fungal richness, pH, and aggregates) governing soil functions to investigate the effects of three biochar rates (C0, no biochar; C1, 4.5 t ha-1 year-1; C2, 13.5 t ha-1 year-1) and two N fertilizer rates (N0, no N fertilizer; N1, 300 kg N ha-1 year-1) on individual soil ecosystem functions and soil multifunctionality. Our results showed that biochar and N fertilizer application increased soil organic carbon (SOC) by 20-58 % and total N content by 9.3-15 % and had a varied effect (but mainly positive) on the activity of enzymes associated with soil carbon, N, and phosphorus cycling. Different application rates of biochar and N fertilizer had no influence on soil DNA concentrations, but did change soil microbial diversity, soil aggregation, and pH. The carbon storage function (SOC content) of soils is an important predictor of multifunctionality. Long-term biochar and N fertilizer application indirectly explained soil multifunctionality by altering soil pH, whereas bacterial and fungal diversity and soil aggregates did not play significant roles in explaining soil multifunctionality. These findings suggest that the application of biochar and N fertilizer can enhance soil multifunctionality by directly improving the individual functions [soil carbon sequestration (SOC content)] and decreasing soil pH in alkaline paddy fields.