Enzymatic hydrolysis of milk protein by complex enzyme mixture of alcalase and neutrase: Kinetic model and hydrolysis control

In order to achieve accurate control of the hydrolysis of cow milk protein, a kinetic model for the hydrolysis of milk protein by a complex enzyme composed of alcalase and neutrase was established. The kinetic model was degree of hydrolysis and the kinetic constants of k 2 , k d and c 0 were 23.752 min −1 , 3.545 min −1 and 1.495 × 10 −3 g/L, respectively. The relationship between hydrolysis conditions, DH and molecular weight was further investigated and quantified, which contributes to the quality control of protein hydrolysates and also provides a theoretical basis for the control of milk protein hydrolysis by complex enzyme.