Rapid detection of monkeypox virus and differentiation of West African and Congo Basin strains using endonuclease restriction-mediated real-time PCR-based testing

A novel multiplex ET-PCR assay that integrates PCR amplification, restriction endonuclease cleavage and real-time fluorescence detection was devised to diagnose MPXV infection and distinguish the Congo Basin and West Africa MPXV strains.