Bosea beijingensis sp. nov., Telluria beijingensis sp. nov. and Agrococcus beijingensis sp. nov., isolated from baijiu mash

The baijiu fermentation environment hosts a variety of micro-organisms, some of which still remain uncultured and uncharacterized. In this study, the isolation, cultivation and characterization of three novel aerobic bacterial strains are described. The cells of strain REN20 T were Gram-negative, strictly aerobic, motile and grew at 26–37 °C, at pH 6.0–9.0 and in the presence of 0–5.0 % (w/v) NaCl. The cells of strain REN29 T were Gram-negative, strictly aerobic, motile and grew at 15–30 °C, at pH 6.0–9.0 and in the presence of 0–10.0 % (w/v) NaCl. The cells of strain REN33 T were Gram-positive, strictly aerobic, motile and grew at 15–37 °C, at pH 5.0–10.0 and in the presence of 0–7.0 % (w/v) NaCl. The digital DNA–DNA hybridization and average nucleotide identity by orthology values between type strains in related genera and REN20 T (20.3–36.8 % and 79.8–89.9 %), REN29 T (20.3–36.8 % and 74.5–88.5 %) and REN33 T (22.6–48.6 % and 75.8–84.2 %) were below the standard cut-off criteria for the delineation of bacterial species, respectively. Based on polyphasic taxonomy analysis, we propose three new species, Bosea beijingensis sp. nov. (=REN20 T =GDMCC 1.2894 T =JCM 35118 T ), Telluria beijingensis sp. nov. (=REN29 T =GDMCC 1.2896 T =JCM 35119 T ) and Agrococcus beijingensis sp. nov. (=REN33 T =GDMCC 1.2898 T =JCM 35164 T ), which were recovered during cultivation and isolation from baijiu mash.