Supplementary Figure 2 from Loss of SMAD4 Promotes Colorectal Cancer Progression by Recruiting Tumor-Associated Neutrophils via the CXCL1/8–CXCR2 Axis

Supplementary Figure 2. A, Screening with a series of kinase inhibitors treated for 24 hours. qRT-PCR analysis for CXCL1 (top) and CXCL8 (bottom). B, Chemotactic responses of neutrophils toward the human recombinant CXCL1 and CXCL8. Mean; bars, {plus minus} SD. *P < 0.05 by Student's t-test. C, Neutrophils were isolated from peripheral blood and from CRC specimens of the same patients (n = 6) by FACS with CD45, CD66b, CD14, CD15, and CD16.