翻译(生物学)
核糖体
信使核糖核酸
细胞生物学
起始因子
真核翻译
蛋白质亚单位
生物
真核小核糖体亚单位
平移移码
真核核糖体
蛋白质生物合成
计算生物学
核糖核酸
遗传学
基因
作者
Christopher P. Lapointe,Rosslyn Grosely,Alex G. Johnson,Jinfan Wang,I.S. Fernandez,Joseph D. Puglisi
标识
DOI:10.1073/pnas.2017715118
摘要
Significance SARS-CoV-2 is the causative agent of the COVID-19 pandemic. A molecular framework for how the virus manipulates host cellular machinery to facilitate infection is needed. Here, we integrate biochemical and single-molecule strategies to reveal molecular insight into how NSP1 from SARS-CoV-2 inhibits translation initiation. NSP1 directly binds to the small (40S) subunit of the human ribosome, which is modulated by human initiation factors. Further, NSP1 and mRNA compete with each other to bind the ribosome. Our findings suggest that the presence of NSP1 on the small ribosomal subunit prevents proper accommodation of the mRNA. How this competition disrupts the many steps of translation initiation is an important target for future studies.
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