乳酸链球菌素
防腐剂
肽聚糖
化学
金黄色葡萄球菌
脂质Ⅱ
食品科学
生物化学
细菌细胞结构
细胞壁
细菌
抗菌剂
生物
有机化学
遗传学
作者
Yuping Ning,Linlin Hou,Mengge Ma,Mingrui Li,Zeguo Zhao,Dongchun Zhang,Zhixin Wang,Yingmin Jia
标识
DOI:10.1016/j.lwt.2022.113145
摘要
Sucrose laurate (SL) is a dual-functional additive with emulsifying and antibacterial activities. The synergistic antibacterial mechanism of SL and nisin were studied against Staphylococcus aureus. Alkaline phosphatase and peptidoglycan assays showed that SL and nisin could synergistically disrupt the integrity of cell wall by blocking the synthesis of peptidoglycan. Fluorescence spectroscopy and flow cytometry analysis revealed that SL and nisin synergistically depolarized the membrane potential, inhibited the Na+K+ and Ca2+Mg2+-ATPase activities on cell membrane, triggered K+ leakage, and damaged the permeability and integrity of cell membrane. SEM and TEM observations further exhibited obvious morphological changes, structural disruption and cytoplasmic leakage. Gel retardation and fluorescence spectroscopy analysis suggested that SL and nisin synergistically influenced the protein and genomic DNA. Finally, the synergistic preservative effect of SL and nisin was evidenced in real food system of milk beverage. This research could provide the insights into broadening the application of SL in food industry.
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