姜黄素
化学
鼻腔给药
药理学
槲皮素
生物利用度
纳米载体
渗透
色谱法
药物输送
抗氧化剂
有机化学
生物化学
医学
膜
作者
Gustavo Richter Vaz,Adryana Clementino,Evgenia Mitsou,Elena Ferrari,Francesca Buttini,Cristina Sissa,Aristotelis Xenakis,Fabio Sonvico,Cristiana Lima Dora
出处
期刊:Pharmaceutics
[MDPI AG]
日期:2022-01-14
卷期号:14 (1): 194-194
被引量:13
标识
DOI:10.3390/pharmaceutics14010194
摘要
The nose-to-brain delivery of neuroprotective natural compounds is an appealing approach for the treatment of neurodegenerative diseases. Nanoemulsions containing curcumin (CUR) and quercetin (QU) were prepared by high-pressure homogenization and characterized physicochemically and structurally. A negative (CQ_NE−), a positive (CQ_NE+), and a gel (CQ_NEgel) formulation were developed. The mean particle size of the CQ_NE− and CQ_NE+ was below 120 nm, while this increased to 240 nm for the CQ_NEgel. The formulations showed high encapsulation efficiency and protected the CUR/QU from biological/chemical degradation. Electron paramagnetic resonance spectroscopy showed that the CUR/QU were located at the interface of the oil phase in the proximity of the surfactant layer. The cytotoxicity studies showed that the formulations containing CUR/QU protected human nasal cells from the toxicity evidenced for blank NEs. No permeation across an in vitro model nasal epithelium was evidenced for CUR/QU, probably due to their poor water-solubility and instability in physiological buffers. However, the nasal cells’ drug uptake showed that the total amount of CUR/QU in the cells was related to the NE characteristics (CQ_NE− > CQ_NE+ > CQ_NEgel). The method used allowed the obtainment of nanocarriers of an appropriate size for nasal administration. The treatment of the cells showed the protection of cellular viability, holding promise as an anti-inflammatory treatment able to prevent neurodegenerative diseases.
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