Electrochemical nano-sensing interface for exosomes analysis and cancer diagnosis

Exosomes are a class of the nanosized extracellular vesicles, which have emerged as representative liquid biopsy biomarkers. To date, the electrochemical nanosensors are of great significance in the exosome detection with the advantages of easy operation, high accuracy and reliable repeatability. Especially, the growing field of nano interface has provided the electrochemical sensing platforms for the accurate exosomes analysis. The incorporation of multiple nanomaterials can take advantages and synergistic properties of functional units. So, based on the integration of with nanomaterial-based signal transduction and specific biorecognition, the nano-sensing interface provides excellent electrochemical features owing to rapid mass transport and excellent conductivity. The nano-sensing interface with a wide variety of morphologies and structure also provides the large active surface area for the immobilization of bio-capturing agents. Furthermore, through the design of nanostructured electrode array, the efficiency of transducer can be greatly improved. It should be noticed that the elaboration of a proper sensor requires the profound knowledge of the nano-sensing interface. Therefore, this article presents a review of the recent advance in exosomes detection based on the electrochemical nano-sensing interface, including electrochemical analysis principles, exosome sensing mechanisms, nano-interface construction strategies, as well as the typical diagnosis application. In particular, the article is focused on the exploration of the various electrochemical sensing performance of nano-interface in the exosome detection. We have also prospected the future trend and challenge of the electrochemical nano-sensing interface for exosomes analysis in clinical cancer diagnosis.