An expriment was conducted to determine the activities of pepsin and trypsin-like enzyme in the larvae of Litopenaeus vannamei Boone using enzyme analytical method.The results indicated that the activities of pepsin and trypsinˉlike enzyme increased with the growth of larval prawn:zoea stagemysis stagepost larval stage.The activities of trypsin-like enzyme were about5times higher than that of pepsin during early ontogeny of Litop Penaeus vannamei Boone.Duringfeedinghabits changing,the activities of pepsin and trypsin-like enzyme in the postlarval stage increased the most,and the activities of trypsin-like enzyme increased higher than that of pepsin.