Extraction,Purification and Determination of Polysaccharides from Cultured Mycelia of Cordyceps Militaris

Objective To establish a method for extraction,purification and determination of polysaccharides from cultured mycelia of Cordyceps militaris. Methods The best condition for extraction of polysaccharides was confirmed by orthogonal experiment,phenol-sulphuric acid was used to determine the contents of polysaccharides,and Sephadex G-100 was used to purify polysaccharides by molecular weight. Results The whole concentration of polysaccharides was 1.36% by water extraction and EtOH precipitation;five compositions from polysaccharide CP-1 was obtained by gel-filtration;and the main monosaccharide of polysaccharide CP-1 was identified as glucose by being hydrolyzed with sulphuric acid. Conclusion The method is simple,reproducible,accurate and reliable,which is fit for extractration,purification and determination of polysaccharides in cultured mycelia of Cordyceps militaris.