Optimization of capsanthin extraction by ultrasonic-microwave synergistic extraction by response surface

Capsanthin,a kind of natural pigment is widely used in food handing,cosmetics manufacturing and pharmaceuticals industry due to its bright color and innoxious.Extraction solvent was 95% ethanol,hot red peppers was the raw material and ultrasonic-microwave synergistic extraction technology was applied in this paper.The main influencing factors,such as extraction temperature,extraction time,solid to liquid ratio,ethanol concentration,ultrasonic time,microwave time was studied.On the basis of single factor experiments,the optimum conditions for the extraction of Capsanthin from Hot red pepper were obtained by Center-united experiment design with 3 factors and 3 levels.According to quadratic regression analysis,the main influencing factors to extraction rate was obtained.Response surface and contour was firstly graphed with extraction rate as the response value.The results showed that the optimum conditions of capsanthin extraction were as follows:the ratio of material to solvent of 1 ∶23,ultrasonic time of 21min,microwave time of 11min,extraction twice.The yield of capsanthin was maximal in this condition.Under such conditions the capsanhtin absorbance(yield)could be up to 0.626.