Infection of human foreskin fibroblasts by lentivirus vector

Objective To investigate the effective and stable transduction of human foreskin fibroblast by lentivirus vector,which can provide a basis for induced pluripotent stem cell research.Methods Human foreskin fibroblast was isolated and cultured with the technique of 0.1% collagenase I digesting.Lentivirus with green fluorescent protein infected cells with three gradients were added into DMEM.Three groups of cells with multiply of infection were added respectively enhanced infection solution after being infected for 48 hours.The expression of green fluorescent proteins and infection efficiency were observed with inverted microscope.Results The model of human foreskin fibroblast was successfully established.The best multiply of infection for lentivirus infecting human foreskin fibroblast was 20,which could achieve 80% infection efficiency.However,enhanced infection solution a kind of infected reagents of polycation was need added in DMEM in order to improve the infection efficiency of lentiviral vectors.Conclusion Lentiviru is easy to infect human foreskin fibroblast and demonstrates a high infection efficiency of human foreskin fibroblast,which is a new vector for iPS technology research.