分区(防火)
细胞生物学
内体
细胞质
细胞外
受体
共焦显微镜
亚细胞定位
细胞内
免疫荧光
信号转导
伤亡人数
共焦
扫描电镜
生物
化学
免疫学
抗体
生物化学
计算机科学
酶
几何学
数学
超分辨率
人工智能
图像(数学)
作者
Harald Husebye,Sarah Doyle
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 65-77
被引量:1
标识
DOI:10.1007/978-1-4939-3335-8_4
摘要
Toll-like receptors (TLR) survey the extracellular space, cytoplasm, and endosomal compartments for signs of infection or tissue injury. Over the past decade, it has become evident that TLR activation and signal transduction can be regulated by subcellular compartmentalization of both the receptors and their downstream signaling components. Immunofluorescence and/or overexpression of fluorescently "tagged"' proteins teamed with confocal microscopy presents a powerful technique for studying the spatial organization of TLRs, their signaling mediators, and the dynamic processes they activate. This chapter details the common methods for determining the subcellular location of TLRs in both live and fixed cells.
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