Micropatterned fabrication of chitosan-based thermoresponsive membranes for improving cell adhesion and gene expression

A simple, rapid, and economical method to fabricate micropatterned thermoresponsive chitosan membranes was developed. Porous polystyrene films were prepared by liquid-induced phase separation. The size of pores on polystyrene films could be regulated by adjusting the composition of coagulation bath and changing the solvent evaporation rate. Subsequently, chitosan-based thermoresponsive membranes with island protrusions were fabricated using porous polystyrene films as templates. The effects of the micropatterns on the behaviors of mouse fibroblast L929 were investigated. The presence of micropatterns altered the cell cycle distribution and enhanced the gene expression of cyclin D1 and integrin β1. The micro-convex surface could promote the adhesion and proliferation of L929 cells. These results provided valuable guidance to design appropriate topographic surfaces for tissue engineering applications.