Culturing and Identifying the Human Dendritic Cells in Vitro

Objective: Explore the experiment method for cultured and identified the human dendritic cells in vitro. Simultaneously, the study would provide requirement for DC research.. Methods :Dendritic cells were isolated from human peripheral blood by Ficoll-Hypaque and Precoll, purified by the way of Panning, and cultured in the medium containing GM-CSF (granulocyte/macrophage colony- stimulating factor, 100μg/mL) and IL-4 (interleukin-4, 10μg/mL), and a large of dendritic cells were harvested. Then they were identified by immunohisto- chemical methods. Results: Dendritic cells had dendritic prominency in 3rd day after harvested viewed by scan electromicroscope. After 7th day, dendritic cells larged and had sheet-like veils or lamel-lipodia on the surface of cell. They behaved the positive staining of S-100 protein antibody. Conclusion :Dendritic cells could be isolated from human peripheral blood and cultured a large of dendritic cells in vitro.